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Dokumenty

A0545

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody

Synonim(y):

Anti Rabbit, Anti Rabbit Antibody, Anti Rabbit Igg, Anti-Rabbit Igg, Goat Anti Rabbit IgG Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Goat Anti Rabbit Igg

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About This Item

Numer MDL:
MFCD00162788
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

Poziom jakości

300

białko sprzężone

peroxidase conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

reaktywność gatunkowa

rabbit

spodziewany brak reakcji z

human

metody

direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Specyficzność

Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.

Immunogen

Purified rabbit IgG

Zastosowanie

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.

Działania biochem./fizjol.

IgGs (immunoglobulins) modulate several immune responses.It mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Uwaga dotycząca przygotowania

Prepared by two-step glutaraldehyde method.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H317

Zwroty wskazujące środki ostrożności

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Klasyfikacja zagrożeń

Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Yu Xiao et al.
Nature, 572(7768), 270-274 (2019-07-11)
Receptor kinases of the Catharanthus roseus RLK1-like (CrRLK1L) family have emerged as important regulators of plant reproduction, growth and responses to the environment1. Endogenous RAPID ALKALINIZATION FACTOR (RALF) peptides2 have previously been proposed as ligands for several members of the
The Immunoglobulins: Structure and Function (1998)
Molecular Genetics of Immunoglobulin (1987)
Teresa Almeida et al.
Molecular biology of the cell, 19(3), 865-876 (2007-12-29)
The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p
Kimberly C Wiegand et al.
BMC cancer, 14, 120-120 (2014-02-25)
Ovarian cancer is now recognized as a number of distinct diseases primarily defined by histological subtype. Both clear cell ovarian carcinomas (CCC) and ovarian endometrioid carcinomas (EC) may arise from endometriosis and frequently harbor mutations in the ARID1A tumor suppressor
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