SAB4200042
Anti-USP7 antibody, Rat monoclonal
clone USP 3D8, purified from hybridoma cell culture
Synonim(y):
Anti-HAUSP, Anti-TEF1, Monoclonal Anti-USP7 antibody produced in rat
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
białko sprzężone
unconjugated
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
primary antibodies
klon
USP 3D8, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen ~130 kDa
reaktywność gatunkowa
human, rat
opakowanie
antibody small pack of 25 μL
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using HeLa cell extracts
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... USP7(7874)
Opis ogólny
Monoclonal Anti-USP7 (rat IgG2a isotype) is derived from the hybridoma USP 3D8 produced by the fusion of mouse myeloma cells (P3X63 AG 8.653) and splenocytes from rat immunized with a peptide.
Ubiquitin specific peptidase 7 (USP7) is a kind of deubiquitylating (DUB) enzyme, which regulates tumor protein p53-mouse double minute 2 homolog (p53-mdm2) pathway. USP7 gene is located on human chromosome 16p13.2.
Specyficzność
Monoclonal Anti-USP7 recognizes human and rat USP7.
Zastosowanie
Monoclonal Anti-USP7 antibody produced in rat may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Działania biochem./fizjol.
Overexpression of the USP7 gene results in p53-mediated apoptosis. Ablation of the USP7 gene expression results in p53 accumulation as opposed to the expected destabilization of p53. This effect has been traced to the ability of USP7 to stabilize mouse double minute 2 homolog (MDM2), a ubiquitin ligase that promotes the degradation of p53. Therefore, USP7 appears to play multiple roles in regulating the p53-MDM2 pathway and in maintaining steady-state levels of p53 in the cell. USP7 is considered for treating hematopoietic tumors due to its role in p53 regulation.
Ubiquitin specific peptidase 7 (USP7) promotes oncogenesis and recognizes specific inhibitors. USP7 is overexpressed in hepatocellular carcinoma (HCC) tissues, which contributes to tumor invasion and proliferation. USP7 is important for osteogenic differentiation of human adipose-derived stem cells (hASCs). It is used for hASC based stem cell therapy and bone tissue engineering. USP7 plays complex roles in DNA regulation, p53 stress response and endosomal protein recycling.
Opis wartości docelowych
USP7 is ubiquitin specific peptidase 7 (herpes virus-associated). It is associated with induction of apoptosis, interspecies interaction between organisms, protein deubiquitination, regulation of protein stability, and ubiquitin-dependent protein cataboli
Postać fizyczna
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Przechowywanie i stabilność
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Clinical Significance of Ubiquitin Specific Protease 7 (USP7) in Predicting Prognosis of Hepatocellular Carcinoma and its Functional Mechanisms
Wang Xujing et al.
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 1742-1742 (2018)
Muyang Li et al.
Molecular cell, 13(6), 879-886 (2004-04-01)
Our previous study showed that ubiquitination of p53 is reversible and that the ubiquitin hydrolase HAUSP can stabilize p53 by deubiquitination. Here, we found that partial reduction of endogenous HAUSP levels by RNAi indeed destabilizes endogenous p53; surprisingly, however, nearly
Structure of USP7 catalytic domain and three Ubl-domains reveals a connector $\alpha$-helix with regulatory role
Kim R et al.
Journal of Structural Biology, 195(1), 11-18 (2016)
Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells
Tang Y, et al.
Stem Cell Research & Therapy, 8(1), 186-186 (2017)
Use of array CGH to detect exonic copy number variants throughout the genome in autism families detects a novel deletion in TMLHE
Celestino-Soper P, et al.
Human Molecular Genetics, 20(22), 4360-4370 (2011)
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