Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Kluczowe dokumenty

Wyświetl całą dokumentację

S1797

Sigma-Aldrich

SOC Medium

For use in transformation

Synonim(y):

Super Optimal broth

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

Wybierz wielkość

100 ML
741,00 zł
10 X 5 ML
1210,00 zł

741,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Wszystkie zdjęcia(1)

Wybierz wielkość

Zmień widok
100 ML
741,00 zł
10 X 5 ML
1210,00 zł

About This Item

Kod UNSPSC:
41106200
NACRES:
NA.85

741,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

klasa czystości

Molecular Biology
for molecular biology

Poziom jakości

500

sterylność

0.2 μm filtered

Formularz

liquid

skład

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

metody

microbiological culture: suitable

Zastosowanie

agriculture

temp. przechowywania

2-8°C

przydatność

nonselective for Escherichia coli
nonselective for coliforms

Powiązane kategorie

Opis ogólny

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector.[1] SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Zastosowanie

SOC Medium has been used for the transformation of DH5α competent E-coli cells. [2] It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest. [3]

Cechy i korzyści

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation
Ta strona może zawierać tekst przetłumaczony maszynowo.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCN8706
SLCL9800
SLCN2539
SLCF7416
SLCG7766

Nie widzisz odpowiedniej wersji?

Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

Wyszukaj dokument COA

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Slide 1 of 4

1 of 4

OF-1 ≥98% (HPLC)

Sigma-Aldrich

SML1184

OF-1

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%

Sigma-Aldrich

B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

LB Broth (Miller) Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Sigma-Aldrich

L3522

LB Broth (Miller)

LB Broth (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7275

LB Broth (Lennox)

Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
Wyświetl wszystkie powiązane dokumenty

Produkty

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protokoły transformacji bakterii

Ogólne protokoły wzrostu komórek kompetentnych i ich transformacji (pobieranie DNA).

Protokoły

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Protokoły komórek kompetentnych

Artykuł techniczny na temat komórek kompetentnych. Transformacja to proces, w którym niektóre bakterie pobierają obcy materiał genetyczny (nagie DNA) ze środowiska.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Protokoły wzrostu drobnoustrojów

Hodowla bakterii E. coli w skali od małych kultur do fermentacji. Uwzględniono protokoły inicjowania kultury starterowej, komórek zawiesinowych, monokultury i posiewu bakteriofaga M13.

Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

      Helpful?

Reviews

No rating value

Active Filters

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej