Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Przejdź do
H8032
Hanahan′s Broth (SOB Medium)
powder microbial growth medium
Synonim(y):
Super Optimal Broth
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
500 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 796,00 zł |
Informacje o tej pozycji
NACRES:
NA.85
UNSPSC Code:
41106200
796,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócgrade
Molecular Biology
Quality Segment
description
recommended for cultivation of recombinant E. coli.
sterility
non-sterile
form
powder
composition
KCl, 0.186 g/L , MgSO4, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.8% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.
Application
Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
Features and Benefits
Hannahan′s Broth (SOB) powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Preparation Note
Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description powder microbial growth medium | description EZMix powder microbial growth medium | description Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture | description Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture. |
| form powder | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| composition KCl, 0.186 g/L , NaCl, 0.5 g/L , Yeast extract, 5 g/L , Tryptone, 20 g/L , MgSO4, 2.4 g/L | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
-