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R2631
Pvu II from Proteus vulgaris
Restriction Enzyme
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
Polecane produkty
klasa czystości
Molecular Biology
for molecular biology
Formularz
buffered aqueous glycerol solution
stężenie
10,000 units/mL
Warunki transportu
wet ice
temp. przechowywania
−20°C
Specyficzność
Recognition sequence: 5′-CAG/CTG-3′
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Zastosowanie
PvuII is a DNA restriction enzyme used for molecular biology methods to cleave the recognition site 5′-CAG/CTG-3′ to generate DNA fragments with blunt termini.
Inne uwagi
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Comment: This enzyme exhibits star activity when used under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Postać fizyczna
Solution in 20 mM Tris-HCl, pH 7.7, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C
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produkt powiązany
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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