For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
- Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
- Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.
Informacje o tej pozycji
biological source
mouse
Quality Segment
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC-480, monoclonal
species reactivity
rat, mouse, human
technique(s)
flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FUT4(2526)
General description
Immunogen
Application
Stem Cell Research
Pluripotent & Early Differentiation
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Zarodkowe komórki macierzyste myszy
Other Notes
Disclaimer
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description clone MC-480, from mouse, PE | description clone 2F1, 0.2 mg/mL, from hamster(Syrian) | description clone C3D-1, Milli-Mark®, from mouse | description clone TRA-2-49/6E, Milli-Mark®, from mouse |
| biological source mouse | biological source hamster (Syrian) | biological source mouse | biological source mouse |
| clone MC-480, monoclonal | clone 2F1, monoclonal | clone C3D-1, monoclonal | clone TRA-2-49/6E, monoclonal |
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Gene Information human ... FUT4(2526) | Gene Information mouse ... Klrg1(50928) | Gene Information human ... FUT4(2526) | Gene Information - |
| conjugate PE | conjugate PE | conjugate FITC conjugate | conjugate FITC conjugate |
| species reactivity rat, mouse, human | species reactivity mouse | species reactivity human | species reactivity human |
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Suggest suitable dilaution of SSEA-1 for flow cutometry.
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