PE0210
Plant Hydrophilic Protein Extraction Kit
Suitable for any plant species or tissue
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About This Item
Kod UNSPSC:
41105500
NACRES:
NA.56
Polecane produkty
temp. przechowywania
−20°C
Opis ogólny
The Plant Hydrophilic Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all hydrophilic proteins from any type of plant species or tissue. The kit includes two reagents, a plant specific protease inhibitor cocktail and a specially formulated reagent to extract hydrophilic proteins (Plant Protein Extraction Reagent Type 1). The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases, and aminopeptidases. It contains pepstatin A, E-64, leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, and 1,10-phenanthroline. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the protein extraction reagent provided. Plant debris is pelleted by centrifugation and the hydrophilic protein extract is collected. The end result is a qualitative soluble hydrophilic protein sample, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the protein extraction reagent provided. Plant debris is pelleted by centrifugation and the hydrophilic protein extract is collected. The end result is a qualitative soluble hydrophilic protein sample, ready for downstream proteomic analysis.
Ilość
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250 mg
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
185.0 °F
Temperatura zapłonu (°C)
85 °C
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M Cilia et al.
Journal of biomolecular techniques : JBT, 20(4), 201-215 (2009-09-02)
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(22), 2179-2183 (2011-06-22)
Orthosiphon aristatus is a traditionally used medicinal plant. In order to study the proteome of the plant, we have developed a simple plant protein extraction method by direct extraction of protein using a modified 2D-gel compatible tris-sucrose buffer followed by
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