OGS91
PSF-CMV-NH2-V5-EKT-NCOI - N-TERMINAL V5 TAG PLASMID
plasmid vector for molecular cloning
Synonim(y):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.85
znacznik
V5 tagged
Postać
buffered aqueous solution
masa cząsteczkowa
size 4280 bp
selekcja bakterii
kanamycin
Pochodzenie replikacji
pUC (500 copies)
Rozszczepienie peptydów
no cleavage
Lokalizacja znacznika peptydowego
N-terminal
Promotor
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
gen reporterowy
none
Warunki transportu
ambient
temp. przechowywania
−20°C
Opis ogólny
Plasmid vector adds a V5 epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the Influenza V5 epitope. The V5 tag coding sequence is GKPIPNPLLGLDST. There is an enterokinase cleavage site (DDDDK) immediately downstream of the V5 tag that can be used to remove the V5 tag from a purified protein. It cleaves after the lysine residue.
Promoter Expression Level: PSF-CMV-NH2-V5-EKT-NCOI - N-terminal V5 tag plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: PSF-CMV-NH2-V5-EKT-NCOI - N-terminal V5 tag plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Zastosowanie
Molecular cloning vector has been designed to allow the addition of a peptide tag to the end of a protein of interest using standard cloning techniques.Multiple Cloning Site Notes:
There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.
The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.
There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.
The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.
Sekwencja
To view sequence information for this product, please visit the product page
Komentarz do analizy
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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