OGS539
PSF-TEFI-TPI1-BLAST - BLASTICIDIN RESISTANT YEAST VECTOR
plasmid vector for molecular cloning
Synonim(y):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.85
Postać
buffered aqueous solution
masa cząsteczkowa
size 6880 bp
selekcja bakterii
kanamycin
Pochodzenie replikacji
2Micron
pUC (500 copies)
Rozszczepienie peptydów
no cleavage
Promotor
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
gen reporterowy
none
Warunki transportu
ambient
temp. przechowywania
−20°C
Opis ogólny
PSF-TEFI-TPI1-BLAST - Blasticidin resistant yeast vector is a blasticidin resistant Saccharomyces cerevisiae expression plasmid. This expression vector contains the yeast EF1-Alpha promoter to drive transgene expression. Saccharomyces cerevisiae yeast expression plasmid using the strong constitutive TEF1 promoter to drive protein production from a gene of interest. The plasmid also contains an antibiotic resistance cassette that provides resistance to blasticidin. This cassette is driven from the strong constitutive TPI yeast promoter.
Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).
Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).
Zastosowanie
Cloning in a gene: PSF-TEFI-TPI1-BLAST - Blasticidin resistant yeast vector has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sekwencja
To view sequence information for this product, please visit the product page
Komentarz do analizy
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Temperatura zapłonu (°F)
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Temperatura zapłonu (°C)
Not applicable
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