K1757

Keratinocyte Growth Factor human

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonim(y): hFGF-7, hKGF, FGF-7, KGF
• Numer CAS: 148348-15-6
• Numer MDL: MFCD00212245
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Nazwa produktu: Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• pochodzenie biologiczne: human
• Poziom jakości: 200
• rekombinowane: expressed in E. coli
• Próba: ≥95% (SDS-PAGE and HPLC)
• Formularz: powder
• siła działania: 0.1-15 ng/mL ED₅₀/EC₅₀
• jakość: endotoxin tested
• masa cząsteczkowa: 18.9 kDa
• opakowanie: pkg of 10 and 50 μg
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: 1 EU/μg
• numer dostępu UniProt: P21781
• temp. przechowywania: −20°C
• informacje o genach: human ... FGF7(2252)

Opis

Opis ogólny

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids,[1] belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines[2], mesenchymal cells[3] but absent from normal glial cells and epithelial cell lines.

Zastosowanie

Keratinocyte Growth Factor human has been used:

• as a component of epithelial growth medium[4]
• as a positive control for antigen to determine the activity of the antibody[5]
• to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions[6]

Działania biochem./fizjol.

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium.[7] It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells.[2] KGF contributes to the survival of cells under stress conditions.[3] KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.[3]

Postać fizyczna

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Komentarz do analizy

Determined by a cell proliferation assay using 4MBr-5 cells.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves