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I3909

Sigma-Aldrich

Ionomycin calcium salt

Ionomycin calcium salt
1 of 1 reviewers received a sample product or took part in a promotion

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

Synonim(y):

Calcium Ionophore Ionomycin

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1 ML
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About This Item

Wzór empiryczny (zapis Hilla):
C41H70CaO9
Masa cząsteczkowa:
747.07
Numer WE:
200-664-3
Numer MDL:
MFCD00083634
Kod UNSPSC:
51102829
Identyfikator substancji w PubChem:
329815372
NACRES:
NA.85

1770,00 zł

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pochodzenie biologiczne

Streptomyces conglobatus

Poziom jakości

300

Próba

≥98% (HPLC)

okres trwałości

2 yr

warunki przechowywania

(Tightly closed. )

stężenie

1 mM in DMSO

kolor

colorless

mp

18.4 °C

rozpuszczalność

DMSO: 1 mM

spektrum działania antybiotyku

Gram-positive bacteria

Tryb działania

cell membrane | interferes

Warunki transportu

wet ice

temp. przechowywania

−20°C

ciąg SMILES

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

Klucz InChI

WKRWUYKLUMMAKG-WYGBAUISSA-L

Opis ogólny

Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.

Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.

Zastosowanie

Ionomycin finds application in:
  • Study of calcium signaling and its role in various biological processes
  • Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
  • Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
  • Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
Ionomycin has been used:
  • to induce central demyelination[1]
  • inhibit adrenal bovine TREK-1 channels[2]
  • to regulate cell division of mature human B cells[3]

Działania biochem./fizjol.

Ca2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca2+.
Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.

Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes

Antimicrobial Spectrum: Active against Gram-Positive bacteria
Ionomycin is an ionophoric antibiotic which binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca2+. It is non-fluorescent. It is used to study Ca2+ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.

Cechy i korzyści

  • Ready available solution reduce the need for preparation time
  • Efficient choice for studying calcium signaling and its role in various biological processes
  • Allows for rapid and controlled manipulation of intracellular calcium levels
  • Versatile and widely used in cell biology research

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000206912
0000213730
0000369268
0000334541
0000325568

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
John J Enyeart et al.
American journal of physiology. Cell physiology, 301(3), C619-C629 (2011-05-27)
Bovine adrenocortical cells express bTREK-1 K(+) (bovine KCNK2) channels that are inhibited by ANG II through a Gq-coupled receptor by separate Ca(2+) and ATP hydrolysis-dependent signaling pathways. Whole cell and single patch clamp recording from adrenal zona fasciculata (AZF) cells
Thorsten Schäfer et al.
Allergy, 65(10), 1242-1255 (2010-03-27)
Mast cells are secretory immune cells whose degranulation can provoke acute allergic reactions. It is presently unclear, however, whether an individual mast cell can repeatedly degranulate or turns dysfunctional after a single antigen stimulus. This work thus aims to better
Fabio Arturo Iannotti et al.
British journal of pharmacology, 176(10), 1568-1584 (2018-08-04)
Duchenne muscular dystrophy (DMD), caused by dystrophin deficiency, results in chronic inflammation and irreversible skeletal muscle degeneration. Moreover, the associated impairment of autophagy greatly contributes to the aggravation of muscle damage. We explored the possibility of using non-euphoric compounds present
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Produkty

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

1 Review This action will navigate to reviews.

Questions

1–7 of 7 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Has Ionomycin calcium salt solution, Product No. I3909, been tested for use in Cell Culture applications?

    1 answer

    1. Sigma Aldrich has not tested this material for use in cell culture.  However, several references for use of ionomycin in cell culture applications can be found in the product information sheet (under Documents, above).

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  4. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  5. What is the concentration of the Ionomycin calcium salt solution, Product No. I3909?

    1 answer

    1. It is a 1mM solution or ~ 0.75mg/mL in DMSO.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. Is Product No. I3909 (Ionomycin calcium salt solution) hygroscopic?

    1 answer

    1. Yes, this product is hygroscopic.  It is packaged under an inert gas.  It is recommended to store I3909 under an inert gas and at -20°C protected from light.

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. Boston, MA
    • Reviews 2
    • Votes 2
    5 out of 5 stars.

    works

    We use it for cell culture and it works as intended.

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