This product is tested for the expansion of TF-1 cells. The activity is reported as the ED50 in nanograms per milliliter. The activity in International Units has not been established. The ED50 value is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5166#product-documentation
H5166
Erythropoietin human
EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonim(y):
EPO
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10 μG
3060,00 zł
3060,00 zł
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10 μG
3060,00 zł
About This Item
3060,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
siła działania
≤5.0 ng/mL ED50
jakość
endotoxin tested
masa cząsteczkowa
dimer 36 kDa (glycosylated)
opakowanie
pkg of 10 μg
warunki przechowywania
avoid repeated freeze/thaw cycles
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1 EU/μg
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... EPO(2056)
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Opis ogólny
EPO has been cloned from various species including human, murine, canine, and others. The mature proteins from the various species are highly conserved and exhibit greater than 80% amino acid sequence identity. EPO contains three N-linked glycosylation sites. The glycosylation of erythropoietin is required for the biological activities of erythropoietin in vivo.
Działania biochem./fizjol.
Erythropoietin (EPO), produced primarily by the kidney, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells. EPO produced by kidney cells is increased in response to hypoxia or anemia. The biological effects of erythropoietin are mediated by the erythropoietin receptor, which binds EPO with high affinity and is a potent EPO antagonist.
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.
Uwaga dotycząca przygotowania
Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Komentarz do analizy
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Klienci oglądali również te produkty
Rajasekhar N V S Suragani et al.
Nature medicine, 20(4), 408-414 (2014-03-25)
Erythropoietin (EPO) stimulates proliferation of early-stage erythrocyte precursors and is widely used for the treatment of chronic anemia. However, several types of EPO-resistant anemia are characterized by defects in late-stage erythropoiesis, which is EPO independent. Here we investigated regulation of
François Roubille et al.
Cardiovascular drugs and therapy, 27(4), 315-331 (2013-05-23)
Erythropoietin (EPO) is the main hormone that regulates erythropoiesis. Beyond its well-known hematopoietic action, EPO has diverse cellular effects in non-hematopoietic tissues. It has been shown to inhibit apoptosis by activating pro-survival pathways in the myocardium, to mobilize endothelial progenitor
Claudia S Robertson et al.
JAMA, 312(1), 36-47 (2014-07-25)
There is limited information about the effect of erythropoietin or a high hemoglobin transfusion threshold after a traumatic brain injury. To compare the effects of erythropoietin and 2 hemoglobin transfusion thresholds (7 and 10 g/dL) on neurological recovery after traumatic
Russia Ha-Vinh Leuchter et al.
JAMA, 312(8), 817-824 (2014-08-27)
Premature infants are at risk of developing encephalopathy of prematurity, which is associated with long-term neurodevelopmental delay. Erythropoietin was shown to be neuroprotective in experimental and retrospective clinical studies. To determine if there is an association between early high-dose recombinant
Léon Kautz et al.
Nature genetics, 46(7), 678-684 (2014-06-02)
Recovery from blood loss requires a greatly enhanced supply of iron to support expanded erythropoiesis. After hemorrhage, suppression of the iron-regulatory hormone hepcidin allows increased iron absorption and mobilization from stores. We identified a new hormone, erythroferrone (ERFE), that mediates
Produkty
Read article on hematopoietic cytokines and hematopoiesis
Wymieniono cytokiny, których głównym działaniem wydaje się być stymulacja lub regulacja komórek krwiotwórczych, w tym GM-CSF, G-CSF, M-CSF, interleukiny, EPO i TPO.
Protokoły
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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