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G4166

Sigma-Aldrich

Glucose Isomerase from Streptomyces murinus

≥350 U/g

Synonim(y):

Sweetzyme® IT Extra, D-xylose ketol-isomerase

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About This Item

Numer EC enzymu:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54

2020,00 zł


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pochodzenie biologiczne

Streptomyces sp. (S. murinus)

Poziom jakości

Formularz

powder

aktywność właściwa

≥350 U/g

temp. przechowywania

2-8°C

Opis ogólny

Immobilized glucose isomerase produced from a selected strain of Streptomyces murinus.

Zastosowanie

Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose.[1][2] It has also been used in the synthetic enzymatic pathway for dihydrogen production from sucrose, to catalyze isomerization of glucose to fructose.[2]
Immobilized glucose isomerase produced from Streptomyces murinus was used for the isomerization of xylose.[3] Glucose isomerase is used in the food industry to produce high-fructose corn syrup[4].

Działania biochem./fizjol.

Few anaerobic bacteria, fungi and plants express an intracellular metalloenzyme called D-xylose isomerase (XI). Most bacteria use the enzyme D-xylose isomerase to transform D-xylose to D-xylulose. D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose.[5]
Glucose isomerase has wide variety of industrial applications such as producing high-fructose corn syrup (HFCS) and production of ethanol from hemicelluloses. In addition, it also facilitates the study of structure-function relationships by advanced biochemical and genetic engineering techniques.[6]

Właściwości fizyczne

0.33 g yields an approximate bed volume of 1ml

Definicja jednostki

one unit converts glucose to fructose at an initial rate of 1 μmole per min at standard analytical conditions

Informacje prawne

A product of Novozyme Corp.
Sweetzyme is a registered trademark of Novozymes Corp.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Leonardo de Figueiredo Vilela et al.
Bioresource technology, 128, 792-796 (2012-11-29)
This study presents results regarding the successful cloning of the bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia and its functional expression in Saccharomyces cerevisiae. The recombinant yeast showed to be competent to efficiently produce ethanol from both glucose and
Kripa Rao et al.
Applied biochemistry and biotechnology, 146(1-3), 101-117 (2008-04-19)
Of the sugars recovered from lignocellulose, D-glucose can be readily converted into ethanol by baker's or brewer's yeast (Saccharomyces cerevisiae). However, xylose that is obtained by the hydrolysis of the hemicellulosic portion is not fermentable by the same species of
Dominik Oberthuer et al.
PloS one, 7(6), e33545-e33545 (2012-06-08)
In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that
Hiroki Kitayama et al.
Biochimica et biophysica acta, 1834(12), 2640-2646 (2013-10-08)
Protein crystals form in supersaturated solutions via a nucleation and growth mechanism. The amyloid fibrils of denatured proteins also form via a nucleation and growth mechanism. This similarity suggests that, although protein crystals and amyloid fibrils are distinct in their
Molecular and industrial aspects of glucose isomerase.
Bhosale SH,et al.
Microbiology and Molecular Biology Reviews, 60(2), 280-300 (1996)

Questions

1–2 of 2 Questions  
  1. Is G4166 an immobilized enzyme? If it is, what support is it immobilized on?

    1 answer
    1. This is a Novozyme product, Sweetzyme® IT Extra, which is an immobilized enzyme. It is glutaraldehyde crosslinked on polyethyleneimine, and intended to be used in a continuous fixed bed process (converting high dextrose syrup to high fructose syrup).

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  2. グルコースイソメラーゼ Streptomyces murinus由来(Cat.No G4166) が可溶化できません。どのように溶かすのでしょうか?またどのような溶液だと可能でしょうか?

    1 answer
    1. This is a product of Novozymes, available commercially as Sweetzyme. This is an immobilized enzyme used in a fixed bed process to convert dextrose syrup to fructose syrup. This material is in granular form with a particle size range of 0.3-1.0 mm. This material is not intended to be solubilized.

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