The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.
F4135
Fetal Bovine Serum
Fetal Bovine Serum
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Synonim(y):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wybierz wielkość
100 ML
827,00 zł
500 ML
3640,00 zł
827,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorczePoproś o próbkę
Wybierz wielkość
Zmień widok
100 ML
827,00 zł
500 ML
3640,00 zł
About This Item
pochodzenie biologiczne:
bovine fetus
pochodzenie:
USA origin
sterylność:
sterile-filtered
Warunki transportu:
dry ice
827,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorczePoproś o próbkę
Polecane produkty
pochodzenie biologiczne
bovine fetus
Poziom jakości
sterylność
sterile-filtered
linia produktu
FBS Classic
skład
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
pochodzenie
USA origin
metody
cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable
zanieczyszczenia
≤10 EU/mL endotoxin
Warunki transportu
dry ice
temp. przechowywania
−20°C
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
Opis ogólny
Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation.[1] It also protects cells from oxidative damage and apoptosis.[2] FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.[3]
Zastosowanie
FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
Fetal Bovine Serum has been used:
- as a component of Dulbecco′s Modified Eagles Media (DME, DMEM),[4] Eagle′s Minimum Essential Medium (EMEM),[5] Roswell Park Memorial Institute medium (RPMI-1640),[6] α-modified eagle medium (αMEM) (MEM)[7] and Leibovitz L-15 medium[8]
- as a component of blocking medium for treating brainstem slices[9]
- to improve attachment of dermal explants[10]
Uwaga dotycząca przygotowania
Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.
Komentarz do analizy
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Ta strona może zawierać tekst przetłumaczony maszynowo.
najczęściej kupowane z tym produktem
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
Nie widzisz odpowiedniej wersji?
Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Produkty
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Wapń jest jonowo stabilnym dwuwartościowym kationem o ważnych korzystnych i toksycznych właściwościach w hodowli komórkowej. Jest składnikiem szerokiej gamy pożywek do hodowli komórkowych.
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Zapoznaj się z podstawami stosowania płodowej surowicy bydlęcej (lub cielęcej) do hodowli komórek oraz z tym, jak pozyskiwanie i przepisy wpływają na cenę surowic do testów komórkowych.
Protokoły
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Chroń swoją inwestycję dzięki najlepszym praktykom przechowywania i rozmrażania FBS i innych surowic. Próbki FBS do walidacji w protokole hodowli komórkowej.
Powiązane treści
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
-
Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?
1 answer-
Helpful?
-
-
Recommended storage is listed as -20C; is it safe/ok to store at -80C?
1 answer-
The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
-
-
Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
1 answer-
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Why is serum Heat-Inactivated (HI)?
1 answer-
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
Helpful?
-
-
Does Sigma-Aldrich offer delipidated bovine serum?
1 answer-
At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
