CLLS1045

MCF10A CELLS HER2 (-/-)

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

• Kod UNSPSC: 41106514
• eCl@ss: 32190102
• NACRES: NA.81

Właściwości

• product name: MCF10A CELLS HER2 (-/-),
• pochodzenie biologiczne: human female mammary glands (Source Disease: Fibrocystic Disease)
• numer dostępu OMIM: 164870
• temp. przechowywania: −196°C
• informacje o genach: human ... ERBB2(2064) , HER2(2064)

Opis

Opis ogólny

MCF10A CELLS HER2 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.

This cell line corresponds to ATCC Catalog No. CRL-10317.

Zastosowanie

Cell freezing medium-DMSO 1X, Cat. No. C6164

Media renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Działania biochem./fizjol.

Human epidermal growth factor receptor 2 (HER2) signaling pathway stimulates cell proliferation and survival in the majority of breast cancers. Thus, overexpression of this protein causes breast cancer. Heterodimeric complex of HER2 and phosphatidylinositide 3-kinase (PI3K) is the most potent stimulator of the PI3K/AKT anti-apoptosis pathway. Increased expression of HER2 is also observed in ovarian, colorectal, pancreatic, endometrial and gastric cancers. Trastuzumab, an antibody, works by binding to a domain in the external domain of HER2. This domain is missing in p95, a truncated form of HER2, and hence these cancer cells show resistance to trastuzumab. HER2 protein can be used as a prognostic marker and as a therapeutic option for gynecologic cancers.

Cechy i korzyści

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) knockout on chromosome 17q21.1

Jakość

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Elementy zestawu są też dostępne oddzielnie

• MCF10A CONTROL CELLS 1 vial
• MCF10A CELLS HER2 (-/-) 1 vial

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable