ONE58 cell line human
10092313, human esophagus, Epithelial-like: cells are spindle-shaped with few vacuoles.
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About This Item
Kod UNSPSC:
41106514
Polecane produkty
product name
ONE58 cell line human, 10092313
pochodzenie biologiczne
human esophagus
opis
Human malignant mesothelioma
tryb wzrostu
Adherent
kariotyp
Not specified
morfologia
Epithelial-like: cells are spindle-shaped with few vacuoles.
produkty
Not specified
receptory
Not specified
metody
cell culture | mammalian: suitable
powiązane choroby
cancer
Warunki transportu
dry ice
temp. przechowywania
−196°C
Pochodzenie linii komórkowej
Human malignant mesothelioma
Opis linii komórkowej
This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143
Zastosowanie
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
pożywka hodowlana
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS
Rutyna subkultury
Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Inne uwagi
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
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