A2942

Amphotericin B

solution, suitable for cell culture, BioReagent

• Synonim(y): Amphotericin, Fungizone
• Wzór empiryczny (zapis Hilla): C₄₇H₇₃NO₁₇
• Numer CAS: 1397-89-3
• Masa cząsteczkowa: 924.08
• Numer MDL: MFCD00877763
• Kod UNSPSC: 12352207
• Identyfikator substancji w PubChem: 24890711
• NACRES: NA.76

Właściwości

• Nazwa produktu: Amphotericin B solution, 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture
• Poziom jakości: 200
• sterylność: 0.1 μm filtered
• linia produktu: BioReagent
• Formularz: liquid
• stężenie: 250 μg/mL in deionized water
• metody: cell culture | mammalian: suitable
• kolor: yellow
• spektrum działania antybiotyku: fungi; yeast
• Tryb działania: cell membrane | interferes
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• ciąg SMILES: [H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(=O)O[Na])O2
• InChI: 1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• Klucz InChI: APKFDSVGJQXUKY-INPOYWNPSA-N

Opis

Opis ogólny

Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain.[1]

Zastosowanie

Amphotericin B solution has been used:

• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice[2]
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea[3]
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line[4]

Działania biochem./fizjol.

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections.[5] It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Amphotericin B is most effective against fungi and yeast.[6] It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules.[6] Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses.[7]

Opakowanie

20,50,100ml

Komponenty

This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.

Przestroga

Amphotericin B remains active for 3 days in culture at 37°C.

Uwaga dotycząca przygotowania

It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Inne uwagi

Keep container tightly closed in a dry and well-ventilated place. Light sensitive.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)