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Merck

A25120

Sigma-Aldrich

DEAE–Sephadex® A-25 chloride form

Synonim(y):

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
47101511
NACRES:
NA.56

Formularz

powder

Poziom jakości

metody

activity assay: suitable

Matryca

cross-linked dextran

grupa aktywna macierzy

functional group

granulacja

40-125 μm (dry)

wielkość porów

~30,000 Da exclusion limit

pH robocze

2-9

pojemność

3-4 meq/g ion exchange capacity

kompatybilność

mode of use mixed weak and strong anion exchange chromatography

Opis ogólny

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Zastosowanie

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Cechy i korzyści

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Informacje prawne

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
William F Loomis et al.
The Journal of biological chemistry, 285(28), 21359-21365 (2010-05-11)
Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty acid metabolism and extracellularly as diazepam binding inhibitor, the precursor of endozepine peptides. Two of these peptides, ODN and TTN, bind to the GABA(A) receptor and modulate its sensitivity to

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