66759

Lactose sulfite medium

suitable for microbiology

• Synonim(y): LS
• Kod UNSPSC: 41171607
• NACRES: NA.21

Właściwości

• okres trwałości: limited shelf life, expiry date on the label
• skład: Cysteine hydrochloride, 0.3 g/L ; Lactose monohydrate, 10 g/L ; Pancreatic digest of casein, 5 g/L ; Sodium chloride, 2.5 g/L ; Yeast extract, 2.5 g/L
• pH końcowe: 7.1±0.2 (25 °C)
• Zastosowanie: microbiology

Opis

Zastosowanie

For the confirmation of Clostridium perfringens.

Uwaga dotycząca przygotowania

Suspend 20,3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense 8 ml portions into tubes with Durham gas collecting tubes for gas detection. Sterilize in autoclave at 121 ºC for 15 minutes. Before using add to each tube 0,5 ml of a 12 g/L solution of sodium metabisulfite and 0,5 ml of a solution of 10 g/L of ferric ammonium citrate. Both solutions have to be freshly prepared and sterilized.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3