11734334001
Roche
Universal Protease Substrate
lyophilized, suitable for detection, pkg of 40 mg (40 μmol; 100mM)
Synonim(y):
Substrate of protease, casein, resorufin-labeled, protease substrate, universal
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About This Item
Kod UNSPSC:
12352204
Polecane produkty
Postać
lyophilized
Poziom jakości
opakowanie
pkg of 40 mg (40 μmol; 100mM)
producent / nazwa handlowa
Roche
stężenie
1 mg/mL
Zastosowanie
detection
Warunki transportu
dry ice
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.
- Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
- Perform highly sensitive protease detection in a homogeneous assay.
Zastosowanie
Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.
Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.
Jakość
Function test: performance-controlled in the assay for proteases.
Uwaga dotycząca przygotowania
Working concentration: Approximately 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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