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PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Synonim(y):

Protease Assay Kit

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.26

Warunki transportu

dry ice

temp. przechowywania

−20°C

Zastosowanie

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Przydatność

Detection limit: approx. 50 ng trypsin

Ilość

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Komentarz do analizy

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Tylko elementy zestawu

Numer produktu
Opis
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H314,H317,H334,H335,H410

Klasyfikacja zagrożeń

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Protokoły

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

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