Przejdź do zawartości
Merck

11663925910

Roche

Mycoplasma PCR ELISA

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352200

zastosowanie

sufficient for 96 reactions

producent / nazwa handlowa

Roche

Warunki transportu

dry ice

temp. przechowywania

−20°C

Specyficzność

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Zastosowanie

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Cechy i korzyści

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Opakowanie

1 kit containing 14 components.

Sekwencja

Chain Length Positive control: 520 bp.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis

  • Lysis Reagent

  • Neutralization Reagent

  • PCR Mix ready-to-use

  • Denaturation Reagent

  • Hybridization Buffer

  • Biotinylated Capture Probe

  • Washing Buffer 10x concentrated

  • Anti-Digoxigenin-POD antibody

  • Conjugate Dilution Buffer

  • TMB Substrate Solution

  • Stop Reagent

  • Positive Control DNA

  • Streptavidin-coated Microplate

  • Adhesive Plate Cover Foils

Zobacz wszystko (14)

Piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Slide 1 of 1

1 of 1

Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi
Margaryan N V, et al.
International Journal of Molecular Sciences, 17(3), 418-418 (2016)
Pengfei Wang et al.
Nature, 593(7857), 130-135 (2021-03-09)
The COVID-19 pandemic has had widespread effects across the globe, and its causative agent, SARS-CoV-2, continues to spread. Effective interventions need to be developed to end this pandemic. Single and combination therapies with monoclonal antibodies have received emergency use authorization1-3
Novel Non-phosphorylated Serine 9/21 GSK3?/a Antibodies: Expanding the Tools for Studying GSK3 Regulation
Tessa G, et al.
Frontiers in Molecular Neuroscience (2016)
Novel Non-phosphorylated Serine 9/21 GSK3?/? Antibodies: Expanding the Tools for Studying GSK3 Regulation
Grabinski T, et al.
Frontiers in Molecular Neuroscience, 9, 123-123 (2016)
Kunal Karve et al.
Cell death & disease, 11(8), 704-704 (2020-08-28)
Alternative splicing contributes to diversification of gene function, yet consequences of splicing on functions of specific gene products is poorly understood. The major transcription factor TCF7L2 undergoes alternative splicing but the biological significance of TCF7L2 isoforms has remained largely to

Produkty

Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma

Protokoły

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Zanieczyszczenie hodowli komórkowych mykoplazmą jest poważnym problemem wpływającym na wiarygodność modeli komórkowych. Test PCR na obecność mykoplazmy jest niedrogą, czułą i specyficzną metodą wykrywania skażenia.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej