11062590001
Roche
DNA Molecular Weight Marker VI
pkg of 50 μg (in 200 μl), solution
Synonim(y):
DNA marker
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About This Item
Kod UNSPSC:
41105335
Polecane produkty
Postać
solution
Poziom jakości
opakowanie
pkg of 50 μg (in 200 μl)
producent / nazwa handlowa
Roche
stężenie
250 μg/mL
kolor
colorless
rozpuszczalność
water: miscible
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
Specyficzność
Specificity: Product has 5′-phosphorylated ends (restriction fragments).
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
Zastosowanie
DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
Sekwencja
As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Definicja jednostki
50 μg = 1A260 unit
Postać fizyczna
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Uwaga dotycząca przygotowania
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Once thawed we recommend further storage at 2 to 8 °C.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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