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10901393001
Roche
Glikogen
from mussels
Synonim(y):
glikogen
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
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1 ML
837,00 zł
837,00 zł
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1 ML
837,00 zł
About This Item
Kod UNSPSC:
41116100
837,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
Poziom jakości
Formularz
solution
opakowanie
pkg of 1 mL (20 mg)
producent / nazwa handlowa
Roche
temp. przechowywania
−20°C
Zastosowanie
This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA).[1][2] As an inert material it may replace tRNAs or sonicated DNAs.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.
Cechy i korzyści
Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.
Contents
Aqueous solution, 20 mg/ml
Contents
Aqueous solution, 20 mg/ml
Komentarz do analizy
Testowane pod kątem braku endonukleaz, aktywności nicowania, egzonukleaz, RNaz, kwasów nukleinowych i proteaz zgodnie z aktualnymi procedurami kontroli jakości.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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