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SCM301

Sigma-Aldrich

3dGRO® Organoid Freeze Medium

Optimized cryopreservation media for multiple organoids cell types.

Synonim(y):

Organoid cryopreservation medium

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About This Item

Kod UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.71

Postać

liquid

metody

cell culture | stem cell: suitable

Powiązane kategorie

Opis ogólny

Organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids address the limitations of existing 2D model systems by providing:

• Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
• Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
• Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.

Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse intestinal organoids, human colon and lung organoids). The media supports higher freeze/thaw viabilities compared to other commercial or homemade organoid freezing media formulations.

Zastosowanie

Optimized cryopreservation media for multiple organoids cell types.
Research Category
Cell Culture

Stem Cell Research

Jakość

Appearance (color): Amber
Osmolality: 250-350 mOsm
pH: 6.8-7.1
Sterility Tested: No Growth/Pass
Bacterial & Fungal Tested: Passed

Przechowywanie i stabilność

Store the 3dGRO Organoid Freeze Medium at -20°C. Aliquot into smaller working aliquots before use to avoid reagent contamination issues.

Informacje prawne

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Produkty

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Różnicowanie in vitro ludzkich komórek iPS w organoidy nabłonka dróg oddechowych na potrzeby badań nad chorobami układu oddechowego

Organoidy płuc są cennymi modelami 3D dla rozwoju ludzkich płuc i chorób układu oddechowego. Protokół różnicowania 3dGRO™ generuje organoidy z ludzkich iPSC w 4 krokach.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

Zobacz wszystko

Protokoły

Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies

How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies

Przewodnik po protokole: Barwienie immunofluorescencyjne organoidów w całości przy użyciu przeciwciał

Jak wybarwiać organoidy? Kompletny protokół krok po kroku do barwienia immunofluorescencyjnego (IF) i immunocytochemicznego (ICC) hodowli organoidów przy użyciu przeciwciał

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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