MABN92

Anti-Iba1/AIF1 Antibody

clone 20A12.1, from mouse

• Synonim(y): Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 20A12.1, monoclonal
• Application: IHC, WB
• Citations: 152

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 20A12.1, monoclonal
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001614
• UniProt accession no.: P55008
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... AIF1(199)

Opis

General description

The allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.

~17 kDa observed

Immunogen

Linear peptide corresponding to human Iba1/AIF1.

Application

Anti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate.

Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate.

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human thymus tissue lysate

Evaluated in Western Blot in human thymus tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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