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Merck
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Dokumenty

MAB3199Z

Sigma-Aldrich

Anti-E-Cadherin Antibody, clone 67A4, Azide Free

clone 67A4, Chemicon®, from mouse

Synonim(y):

CD324, MAB3199

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

67A4, monoclonal

reaktywność gatunkowa

human

opakowanie

antibody small pack of 25 μg

producent / nazwa handlowa

Chemicon®

metody

flow cytometry: suitable
immunofluorescence: suitable

izotyp

IgG1

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CDH1(999)

Powiązane kategorie

Specyficzność

Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Immunogen

T-47D breast carcinoma line.

Zastosowanie

Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting

FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Optimal working dilutions must be determined by end user.

Jakość

Tested

Postać fizyczna

Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Michael K Dame et al.
Laboratory investigation; a journal of technical methods and pathology, 94(2), 222-234 (2013-12-25)
In order to advance a culture model of human colonic neoplasia, we developed methods for the isolation and in vitro maintenance of intact colonic crypts from normal human colon tissue and adenomas. Crypts were maintained in three-dimensional Matrigel culture with
Isao Matsuura et al.
The Journal of biological chemistry, 285(3), 1754-1764 (2009-11-19)
Transforming growth factor-beta (TGF-beta) regulates a wide variety of biological activities. It induces potent growth-inhibitory responses in normal cells but promotes migration and invasion of cancer cells. Smads mediate the TGF-beta responses. TGF-beta binding to the cell surface receptors leads
Ceri E Stewart et al.
Journal of allergy, 2012, 943982-943982 (2012-01-31)
The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma research. Ability to differentiate into goblet
The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation.
Marrero, B; Heller, R
Biomaterials null
Gracia Y Ng et al.
Investigative ophthalmology & visual science, 54(13), 7933-7942 (2013-11-10)
Shp2 protein tyrosine phosphatase mediates a wide variety of receptor tyrosine kinases (RTK) cell signaling. Herein, we investigate the role of Shp2 in corneal morphogenesis and homeostasis. Shp2 was conditionally knocked out (Shp2(cko)) in Krt14-rtTA;tet-O-Cre;Shp2(f/f) triple transgenic mice administrated with

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