The immunogen for this antibody is mitochondrial lysate from Raji lymphoma cells, & the exact target protein has not been characterized. Clone 113-1 is known to recognize a conserved epitope on the outer mitochondrial membrane.
MAB1273
Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
clone 113-1, Chemicon®, from mouse
Synonim(y):
Mitochondrial antibody
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 μL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 2030,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
113-1, monoclonal
Species reactivity:
human
Application:
IF, IHC (p), IP
Citations:
205
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
113-1, monoclonal
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
input
sample type neural stem cell(s)
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
65 kDa
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.
Immunogen
Epitope: surface of intact mitochondria
Application
Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.
Immunohistochemistry:
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.
MAB1273 recognizes a 65 kD protein by immunoprecipitation.
Optimal working dilutions and protocols must be determined by the end user.
1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Immunohistochemistry in paraffin embedded tissues:
Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.
Indirect immunofluorescence:
1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.
MAB1273 recognizes a 65 kD protein by immunoprecipitation.
Optimal working dilutions and protocols must be determined by the end user.
Biochem/physiol Actions
Gives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.
MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.
Reactivity with other species has not been determined
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.
Preparation Note
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Analysis Note
Control
COS7 cells.
COS7 cells.
Routinely evaluated by immunohistochemistry on heart ventricle cells.
Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
Immunohistochemistry(paraffin) Analysis:
Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| clone 113-1, monoclonal | clone 113-1, monoclonal | clone 113-1, monoclonal | clone polyclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form serum |
| species reactivity human | species reactivity human (mitochondria protein) | species reactivity human (mitochondria protein) | species reactivity monkey, human |
| technique(s) immunofluorescence: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable | technique(s) immunocytochemistry: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| MAB1273 | 04053252400391 |
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What is the surface marker antigen for detection?
1 answer-
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Hello, I need to know if this antibody crossreact with rabbit proteins.
1 answer-
The reactivity in rabbits has not been determined. Unfortunately, an alternative option is not available.
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