AB1870
Anti-Osteopontin Antibody
serum, Chemicon®
Synonim(y):
Uropontin, Bone sialoprotein 1, SPP1/CALPHA1 fusion, Urinary stone protein, bone sialoprotein I, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialop
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Wybierz wielkość
100 μL
2910,00 zł
2910,00 zł
Przewidywany termin wysyłki22 kwietnia 2025Szczegóły
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Zmień widok
100 μL
2910,00 zł
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
2910,00 zł
Przewidywany termin wysyłki22 kwietnia 2025Szczegóły
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
mouse, rat, human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SPP1(6696)
Opis ogólny
Osteopontin is a single-chain polypeptide with a molecular weight of approximately 35 kDa. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.
Specyficzność
Expected to cross-react with rat.
Human osteopontin. Does not cross react with osteonectin or human bone sialoprotein II.
Immunogen
Synthetic peptide corresponding to amino acids 75-90 of human osteopontin (SwissProt Accession # P10451; PSKSNESHDHMDDM DD).
Zastosowanie
Immunohistochemistry:
A previous lot of this antibodu was used at a 1:1500 dilution on paraffin-embedded tissue sections.
ELISA:
A previous lot of this antibody was used at a 1:1000 dilution.
Optimal working dilutions must be determined by end user.
A previous lot of this antibodu was used at a 1:1500 dilution on paraffin-embedded tissue sections.
ELISA:
A previous lot of this antibody was used at a 1:1000 dilution.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Osteobiology
Osteobiology
This Anti-Osteopontin Antibody is validated for use in ELISA, IH, IH(P), WB for the detection of Osteopontin.
Jakość
Routinely evaluated by Western Blot on RAW 264.7 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected osteopontin on 10 μg of RAW 264.7 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected osteopontin on 10 μg of RAW 264.7 lysates.
Opis wartości docelowych
~35 kDa
Postać fizyczna
Rabbit polyclonal antiserum in buffer containing no preservatives.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
POSITIVE CONTROL: Human urine (2-5 µg/mL normal concentration).
POSITIVE CONTROL: Human urine (2-5 µg/mL normal concentration).
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Serum osteopontin predicts degree of hepatic fibrosis and serves as a biomarker in patients with hepatitis C virus infection.
Matsue, Y; Tsutsumi, M; Hayashi, N; Saito, T; Tsuchishima, M; Toshikuni, N; Arisawa, T; George, J
Testing null
Nazwin Basheer et al.
European endodontic journal, 6(3), 295-302 (2021-12-31)
The aim of this study was to comparatively evaluate the proliferation, differentiation and mineralization inducing potential of strontium incorporated tetracalcium phosphate cement (STTCP) and mineral trioxide aggregate (MTA) on human dental pulp stem cells (hDPSCs). hDPSCs were cultured from freshly
Warren L Grayson et al.
Tissue engineering. Part A, 14(11), 1809-1820 (2008-07-16)
We describe a novel bioreactor system for tissue engineering of bone that enables cultivation of up to six tissue constructs simultaneously, with direct perfusion and imaging capability. The bioreactor was used to investigate the relative effects of initial seeding density
Mirjam Fröhlich et al.
Tissue engineering. Part A, 16(1), 179-189 (2009-08-15)
We report engineering of half-centimeter-sized bone constructs created in vitro using human adipose-derived stem cells (hASCs), decellularized bone scaffolds, and perfusion bioreactors. The hASCs are easily accessible, can be used in an autologous fashion, are rapidly expanded in culture, and
Sequential application of steady and pulsatile medium perfusion enhanced the formation of engineered bone.
Correia, C; Bhumiratana, S; Sousa, RA; Reis, RL; Vunjak-Novakovic, G
Tissue Engineering: Part A null
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