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556881

Sigma-Aldrich

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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About This Item

Kod UNSPSC:
12352200

Poziom jakości

Formularz

liquid

aktywność właściwa

≥20,000 units/mL

producent / nazwa handlowa

Calbiochem®

warunki przechowywania

OK to freeze

obecność zanieczyszczeń

Endonuclease and RNase, none detected

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Działania biochem./fizjol.

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Ostrzeżenie

Toxicity: Standard Handling (A)

Definicja jednostki

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Postać fizyczna

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Rekonstytucja

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Inne uwagi

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Odwiedź Bibliotekę dokumentów

Nida Ali et al.
Nucleic acids research, 48(2), 847-861 (2019-12-06)
RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated
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Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild
Claudia S Barros et al.
STAR protocols, 3(4), 101735-101735 (2022-10-02)
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then
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