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R1158

Sigma-Aldrich

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

Synonim(y):

RNAse inhibitor

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About This Item

Numer MDL:
MFCD00132187
Kod UNSPSC:
12352200
NACRES:
NA.52

rekombinowane

expressed in E. coli

Poziom jakości

300

klasa czystości

for molecular biology

Postać

buffered aqueous solution

masa cząsteczkowa

~50 kDa

stężenie

20-40 units/μL

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Zastosowanie

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

  • cDNA synthesis from mRNA
  • reverse transcriptase polymerase chain reaction (RT-PCR)
  • in vitro transcription and translation
  • mRNA protection from degradation
  • formaldehyde cross-linked lncRNA purification
  • RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Działania biochem./fizjol.

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Komponenty

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Definicja jednostki

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Postać fizyczna

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Inne uwagi

  • Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
  • This product is for R&D use only, not for drug, household, or other uses.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Lintao Li et al.
Cell cycle (Georgetown, Tex.), 22(4), 476-493 (2022-11-13)
Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected to microCT analysis
Antisense overlapping long non-coding RNA regulates coding arachidonate 12-lipoxygenase gene by translational interference
Sabbir MG, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1866(10), 158987-158987 (2021)
A fine-needle aspiration-based protein signature discriminates benign from malignant breast lesions
Franzen B, et al.
Molecular Oncology, 12(9), 1415-1428 (2018)
Nadia A Hasaneen et al.
American journal of physiology. Lung cellular and molecular physiology, 293(4), L1059-L1068 (2007-08-19)
Angiogenesis is an important feature of airway remodeling in both chronic asthma and chronic obstructive pulmonary disease (COPD). Airways in those conditions are exposed to excessive mechanical strain during periods of acute exacerbations. We recently reported that mechanical strain of
Comparison of in vitro viability methods for Cryptosporidium oocysts
Burgt NHV, et al.
Experimental Parasitology, 187, 30-36 (2018)
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