344289
Freund′s Complete Adjuvant, Modified, Mycobacterium butyricum
This Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum is validated for use in Immunization.
Synonim(y):
Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum
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1 SET
1120,00 zł
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1 SET
1120,00 zł
About This Item
Polecane produkty
opis
RTECS - LS6860000
Poziom jakości
Formularz
liquid
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
kolor
clear to slightly cloudy amber
Warunki transportu
ambient
temp. przechowywania
2-8°C
Opis ogólny
1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.
Contains 10 vials per set, each containing 10 ml of Freund′s complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.
Zastosowanie
Immunization
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
Yellow, oily, bacterial suspension.
Inne uwagi
Composition
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Asp. Tox. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
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