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Sigma-Aldrich

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate

clone 2F3.2, Upstate®, from mouse

Synonim(y):

70 kDa zeta-associated protein, Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

białko sprzężone

FITC conjugate

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

2F3.2, monoclonal

reaktywność gatunkowa

human, rat

producent / nazwa handlowa

Upstate®

metody

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

izotyp

IgG2a

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

Powiązane kategorie

Opis ogólny

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals

Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)

Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Specyficzność

Expected to cross-react with rat.
Recognizes human ZAP-70.

Immunogen

GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.

Zastosowanie

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.
Immunocytochemistry:
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Jakość

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.

Opis wartości docelowych

70 kDa

Postać fizyczna

Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.

Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Protein G Purified

Przechowywanie i stabilność

Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.

Komentarz do analizy

Control
Western Blot: Jurkat cell lysate.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Fortunato Morabito et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 19(21), 5890-5900 (2013-09-17)
To investigate the incidence and clinical relevance of classic and new prognostic markers, IGHV gene mutational status, and chromosomal abnormalities in clinical monoclonal B lymphocytosis (cMBL) compared with Rai stage 0 chronic lymphocytic leukemia (Rai0-CLL). A group of 136 patients
M Szabo et al.
International immunology, 24(2), 79-87 (2011-12-31)
Zeta-chain-associated protein kinase of 70kDa (ZAP-70) kinase is a key regulator in the early steps of TCR signaling but some aspects of its fine regulation are still unclear. From its 31 tyrosine (Y) residues, 11 phosphorylation sites have been identified
Chronic lymphocytic leukaemia: new biological markers for assessing prognosis.
Wagner, Simon D and Cwynarski, Kate
The Hematology Journal : The Official Journal of the European Haematology Association / EHA, 5, 197-201 (2004)
TCR signaling: another Abl-bodied kinase joins the cascade.
Wange, Ronald L
Current Biology, 14, R562-R564 (2004)
Roberta Pascolutti et al.
Cell reports, 27(10), 3049-3061 (2019-06-06)
Adaptor protein 2 (AP2) is a major constituent of clathrin-coated pits (CCPs). Whether it is essential for all forms of clathrin-mediated endocytosis (CME) in mammalian cells is an open issue. Here, we demonstrate, by live TIRF microscopy, the existence of

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