129935

Actinomycin D, 7-Amino-

7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

• Synonim(y): Actinomycin D, 7-Amino-, 7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin
• Wzór empiryczny (zapis Hilla): C₆₂H₈₇N₁₃O₁₆
• Numer CAS: 7240-37-1
• Masa cząsteczkowa: 1270.43
• Numer MDL: MFCD00077295
• Kod UNSPSC: 12352200

Właściwości

• Poziom jakości: 100
• Próba: ≥85% (HPLC)
• Formularz: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic); protect from light
• kolor: red-purple
• moc wejściowa: sample type intact cells
• rozpuszczalność: DMSO: 20 mg/mL; ethanol: 5 mg/mL
• Warunki transportu: ambient
• temp. przechowywania: −20°C
• InChI: 1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)
• Klucz InChI: YXHLJMWYDTXDHS-UHFFFAOYSA-N

Opis

Opis ogólny

7-Aminoactinomycin D is a membrane impermeable fluorescent DNA intercalator. Like its parent compound, Actinomycin D (Cat. No. 114666), 7-AAD has antibacterial properties and growth inhibitory activity against certain leukemias and sarcomas. Useful for flow cytometry and fluorescence microscopy. Used for staining nonviable cells and for identifying apoptotic cells. Compatible with FITC and PE cell surface staining. The absorption/emission maxima are as follows: unbound (ex. 500 nm, em. 672 nm) and DNA bound (ex. 555 nm, em. 655 nm). λ_max 528 nm ± 2; ε_max = 22,700 ± 5,000 M^-1 cm^-1

7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Fluorescent excitation and emission maxima are 500 and 672 nm, respectively. Complexes with DNA and absorbs at 555 nm and emits at 655 nm. Useful in distinguishing early apoptotic cells, which have lost membrane integrity, from apoptotic and live cells. Has been used in DNA analysis and as a probe for chromosome structure and function.

Działania biochem./fizjol.

Primary Target
leukemias and sarcomas

Ostrzeżenie

Toxicity: Highly Toxic (H)

Uwaga dotycząca przygotowania

Further dilute in aqueous buffers just prior to use.

Rekonstytucja

Following reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Inne uwagi

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Schmid, I., et al. 1994. J. Immunol. Methods 170, 145.
Berg, R.J., et al. 1993. Carcinogenesis14, 103.
Chen, F.M. 1992. Biochemistry31, 6223.
Schmid, I., et al. 1992. Cytometry13, 204.
Toba, K., et al. 1992. Cytometry13, 60.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H300
• Zwroty wskazujące środki ostrożności: P264 - P270 - P301 + P310 - P405 - P501
• Klasyfikacja zagrożeń: Acute Tox. 2 Oral
• Kod klasy składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 3