07-1520

Anti-phospho-Grb10 (Ser501/Ser503) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): growth factor receptor-bound protein 10, OTTHUMP00000197988, OTTHUMP00000197989, OTTHUMP00000197990, OTTHUMP00000197991, OTTHUMP00000197992, OTTHUMP00000197993, OTTHUMP00000197994, GRB10 adapter protein, GRB10 adaptor protein, maternally expressed gene 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse, horse, pig
• reaktywność gatunkowa (przewidywana na podstawie homologii): rhesus macaque (based on 100% sequence homology), equine (based on 100% sequence homology), primate (based on 100% sequence homology), porcine (based on 100% sequence homology), human (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_005302
• numer dostępu UniProt: Q13322
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer501/pSer503)
• informacje o genach: human ... GRB10(2887)

Opis

Opis ogólny

Growth factor receptor-bound protein 10 (Grb10) belongs to a small family of structurally related multi-domain adaptor proteins, which are known to be involved with a diverse array of functions, including cellular growth, metabolism, apoptosis, and cell migration. Grb10 is widely, but not uniformly, expressed in mammalian tissues, and there are various known isoforms of Grb10. It is shown to bind to and positively or negatively mediate signals from a number of activated receptor tyrosine kinases, including the insulin (INSR) and the insulin-like growth factor (IGF1R and IGF2R) receptors, in addition to a variety of growth factor receptors and intracellular molecules, in a context-dependent manner. In one study, mTORC1-mediated Grb10 phosphorylation is demonstrated to lead to feedback inhibition of the phosphatidylinositol-3-kinase (PI3K) and the extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways in HEK 293T cells, in vivo. However, many aspects of the Grb10 adapter function remain unclear. Further research is still required to elucidate and validate the interactions and effects of GRB10 with various cellular targets.

Specyficzność

Other homologies: Rat (91% sequence homology).

This antibody recognizes Grb10 phosphorylated at Ser501/Ser503.

Immunogen

Epitope: Phosphorylated Ser501/Ser503.

KLH-conjugated linear peptide corresponding to human Grb10 phosphorylated at Ser501/Ser503.

Zastosowanie

Anti-phospho-Grb10 (Ser501/Ser503) Antibody detects level of phospho-Grb10 (Ser501/Ser503) & has been published & validated for use in WB.

Research Category
Metabolism

Research Sub Category
Glucose/Glycogen Metabolism

Jakość

Evaluated by Western Blot in insulin untreated and treated mouse embryonic fibroblast cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected Grb10 in 10 µg of insulin untreated and treated mouse embryonic fibroblast cell lysates.

Opis wartości docelowych

~66 kDa observed. Uncharacterized bands detected in some lysates. UniProt describes 4 isoforms produced by alternative splicing: Isoform 1 at 62.026 kDa, Isoform 2 at 60.848 kDa, Isoform 3 at 67.231 kDa, and Isoform 4 at 66.487 kDa

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Insulin untreated and treated mouse embryonic fibroblast cell lysates

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable