推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3N20, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 265.35 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
rat
包裝
antibody small pack of 25 μL
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
同型
IgG
表位序列
N-terminus
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CREBBP(1387)
一般說明
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3N20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CREB-binding protein (CBP). It targets an epitope within 25 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human CREB-binding protein (CBP).
應用
Quality Control Testing
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CREB-binding protein (CBP) in MCF-7 cells.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound CREB-binding protein (CBP) peptide with a KD of 1.3 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CREB-binding protein (CBP) in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CREB-binding protein (CBP) in MCF-7 cells.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound CREB-binding protein (CBP) peptide with a KD of 1.3 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CREB-binding protein (CBP) in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
CREB-binding protein (UniProt: Q92793; also known as EC: 2.3.1.48, Histone lysine acetyltransferase CREBBP, Protein-lysine acetyltransferase CREBBP) is encoded by the CREBBP (also known as CBP) gene (Gene ID: 1387) in human. CBP, a homolog of p300, serves as a transcriptional co-activator of various sequence-specific transcription factors that are involved in a wide array of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis. It possesses intrinsic acetyltransferase activity by which it acetylates histones, giving specific tag for transcription activation. It can also acetylate non-histone proteins, such as DExD-box helicase 21, interferon regulatory factor 2, and nuclear receptor coactivator 3. CBP facilitates the transcription of active genes by acting as adapter protein bridging DNA binding transcription factors to the basal transcription machinery and by loosening chromatin through histone tail acetylation. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CBP and p300 share overall 63% amino acid sequence identity and about 90% homology in their histone acetyltransferase domain (aa 1323-1700). Structurally, CBP contains several domains: a receptor-interacting domain (RID); KIX binding site of CREB; a bromo domain (aa 1103-1175); three cysteine and histidine-rich domains, a glutamine-and proline-rich domain (QP), and a steroid receptor co-activator-1 interaction domain (SID). Methylation of the KIX domain by coactivator associated arginine methyltransferase 1 (CARM1) blocks its association with CREB that results in the blockage of CREB signaling. Phosphorylation of CBP by IKK- at serine 1382 and 1386 promotes cell growth by switching the binding preference of CBP from TP53 to NF-kB. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Das, C., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(12); E1072-E1081; Song, C., et al. (2017). Genes Dev. 31(13); 1370-1381; Karamouzis, MV., et al. (2007). Cell Res. 17(4); 324-332).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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