This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.
F1804
单克隆抗-FLAG® M2 小鼠抗
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
别名:
flag抗体, 抗 ddddk, 抗 dykddddk
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所有图片(1)
About This Item
UNSPSC代码:
12352203
NACRES:
NA.32
价格与库存信息目前不能提供
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生物来源
mouse
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
纯化方式
affinity chromatography (affinity tag purification)
种属反应性
all
浓度
1 mg/mL
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
wet ice
储存温度
−20°C
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一般描述
小鼠抗-FLAG M2抗体，可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。
纯化方法 - 亲和标签纯化
纯化方法 - 亲和标签纯化
特异性
结合位点：N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
免疫原
FLAG；肽序列DYKDDDDK
应用
外形
在磷酸盐缓冲液中含有50%甘油。
制备说明
在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液（TBS），pH 8.0
其他说明
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?
Barbara Lipert
PLoS ONE (2017)
ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
Yumin Qiu et al.
Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as
商品
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。
相关内容
蛋白质与核酸相互作用试剂，以及用于研究蛋白质-RNA、蛋白质-DNA、蛋白质-蛋白质相互作用和相关应用的资源。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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Is this Ab suitable for ELISA?
1 answer-
Helpful?
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Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci
1 answer-
Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
https://www.nature.com/articles/s41467-019-12187-5.pdfHelpful?
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モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
1 answer-
An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdfHelpful?
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