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重組細胞
expressed in E. coli
化驗
≥65% (SDS-PAGE)
形狀
aqueous solution
分子量
37 kDa
包裝
pkg of 100 μg
NCBI登錄號
運輸包裝
dry ice
儲存溫度
−70°C
一般說明
生物素连接酶BirA（GenBank 登记号AP012306 (3602298-3603260)）氨基酸 2-321（末端）具有 His-FLAG-标签，分子量 37.1kDa，表达于大肠杆菌细胞表达系统中。
應用
可用于研究酶动力学、抑制剂筛选和选择性分析。
生化／生理作用
生物素连接酶 BirA 控制 大肠杆菌 的生物素合成。它将生物素送入新陈代谢。作为一种同源二聚体，它负性调控生物素合成操纵子。BirA还可以与乙酰辅酶A羧化酶的生物素受体蛋白一起催化翻译后生物素化。 它可以使人类组蛋白生物素化。
外觀
溶于 50mM Tris-HCl，pH 8.0，50mM NaCl，150 mM 咪唑，3mM DTT 和 5％甘油中。
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Vandana Chakravartty et al.
Journal of bacteriology, 194(5), 1113-1126 (2012-01-03)
Transcription of the Escherichia coli biotin (bio) operon is directly regulated by the biotin protein ligase BirA, the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein
Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3.
Kobza K, et al.
Bmb Reports, 41, 310-315 (2008)
Functional versatility of a single protein surface in two protein:protein interactions.
Adikaram PR and Beckett D
Journal of Molecular Biology, 419, 223-233 (2012)
Hema Chandra Kotamarthi et al.
Cell reports, 30(8), 2644-2654 (2020-02-27)
ATP-powered unfoldases containing D1 and D2 AAA+ rings play important roles in protein homeostasis, but uncertainty about the function of each ring remains. Here we use single-molecule optical tweezers to assay mechanical unfolding and translocation by a variant of the
Yifeng Li et al.
Protein expression and purification, 82(1), 162-167 (2012-01-10)
The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor
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