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Merck

B4639

Sigma-Aldrich

生物素

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

别名:

D-生物素, Bios II, 维生素B7, 维生素H, 辅酶 R

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About This Item

经验公式(希尔记法):
C10H16N2O3S
CAS号:
分子量:
244.31
Beilstein:
86838
EC號碼:
MDL號碼:
分類程式碼代碼:
12352201
eCl@ss:
34058011
PubChem物質ID:
NACRES:
NA.21
价格与库存信息目前不能提供

產品線

BioReagent

品質等級

化驗

≥99%

形狀

powder

分子量

Mw 244.31 g/mol

技術

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

顏色

white to off-white

mp

231-233 °C (lit.)

溶解度

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

儲存溫度

2-8°C

SMILES 字串

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI 密鑰

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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一般說明

生物素又称B复合维生素。这是一种双环化合物,分子量244.31 Da。生物素是哺乳动物羧化酶的重要辅助因子。它在细胞中以游离和结合混合物形式存在。[1]

應用

生物素已用于:
  • 作为体外培养系统中的M199培养基补充剂[2]
  • 作为分离皮质少突胶质细胞前体(OPC)的SATO无血清培养基成分[3]
  • 脂肪酸从头合成的生化前体培养[4]
  • 作为聚集培养基组分[5]

生化/生理作用

对于氨基酸、能量代谢和脂肪酸合成有重要作用的必要维生素。它是四种哺乳类动物羧化酶中的辅基,促进二氧化碳的结合与输送。

相關產品

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. If I would like to make 1M biotin stock (B4639), what would be the best way?

    1 answer
    1. A 1 M solution of biotin would require a concentration of 244 mg/mL. The maximum solubility tested for this compound is 50mg/ml in 2M NaOH or NH4OH, which results in a 204 mM solution. Similar rates have been reported for DMSO. Heat and agitation may improve the solubility. However, a precipitate may form upon cooling. A search of the literature did not reveal a more efficient solvent for Biotin. The preparation of a 1M solution would have to be determined experimentally.

      Helpful?

  4. Hello, How can I reconstruct biotin powder (B4639)? which solvent? and how can I prepare 50mM biotin solution for 1 ml final solution?

    1 answer
    1. This product is soluble in 50 mg/ml 2 M NH4OH.

      Helpful?

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