推荐产品
用途
sufficient for 5 labeling reactions
品質等級
包裝
kit of 1 (5 components)
製造商/商標名
Roche
技術
ELISA: suitable
immunoblotting: suitable
儲存溫度
2-8°C
一般說明
用生物素标记蛋白。
待标记蛋白的游离氨基酸可通过形成稳定的氨基键,与D-生物素基--ε-氨基己酸-N-羟基-琥珀酰亚胺酯(生物素-7-NHS)反应。在Sephadex G-25柱上对未反应的生物素-7-NHS进行分离。
待标记蛋白的游离氨基酸可通过形成稳定的氨基键,与D-生物素基--ε-氨基己酸-N-羟基-琥珀酰亚胺酯(生物素-7-NHS)反应。在Sephadex G-25柱上对未反应的生物素-7-NHS进行分离。
應用
生物素蛋白标记试剂盒用于生产变性的生物素化牛血清白蛋白(d-BSA),以包覆碲化镉(CdTe)量子点(QD)缀合物。
该试剂盒适用于通过生物素标记蛋白进行免疫化学、免疫印迹和ELISA。
该试剂盒适用于通过生物素标记蛋白进行免疫化学、免疫印迹和ELISA。
包裝
1个试剂盒包含5种组分。
品質
功能测试。
準備報告
工作溶液:溶液制备
- 封闭液:将封闭剂瓶(绿色螺旋盖)中的内容物溶于300 ml双蒸水中。
- PBS溶液:将PBS瓶(蓝色螺旋盖)中的内容物溶于300 l双蒸水中。
- 生物素-7-NHS溶液:溶液应在标记前现配。加到包含250μI生物素-7-NHS DMSO溶液的反应样品瓶的内容物中,并将溶液涡旋数次。得到的生物素-7-NHS溶液浓度为20 mg/ml。对于标记混合物,生物素-7-NHS溶液的体积不应超过总反应体积的5%(例如,最多可向1 ml蛋白溶液中加50 μl生物素-7-NHS溶液)。
其他說明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- Blocking Reagent (green screw cap)
- Phosphate Buffered Saline (PBS) (blue screw cap)
- D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
- Dimethylsulfoxide (DMSO) (red screw cap)
- Sephadex G-25 Columns
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
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