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SAE0092

Sigma-Aldrich

变溶菌素 来源于球孢链霉菌 ATCC 21553

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

别名:

Mutanolysin

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About This Item

CAS号:
55466-22-3
MDL编号:
MFCD00131635
UNSPSC代码:
12352202
NACRES:
NA.54

生物来源

Streptomyces sp. (Streptomyces globisporus ATCC 21553)

质量水平

200

表单

lyophilized powder

比活

≥4000 units/mg protein (biuret)

分子量

23 kDa
 ~18.5 kDa

特点

DNA free

浓度

≥40% protein

技术

nucleic acid detection: suitable

适用性

suitable for cell lysis

应用

life science and biopharma

运输

wet ice

储存温度

−20°C

相关类别

应用

为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
纯化的变溶菌素SAE0092已经过严格的质量控制测试，以确保通过35个循环对16S和18S rDNA（使用通用引物对）进行PCR扩增时，不存在可检测水平的污染DNA。
变溶菌素是一种胞壁溶解酶，适合分离来自革兰氏阳性菌（如李斯特菌、乳杆菌、乳球菌）(8)和其他仅用溶菌酶难以裂解的细菌。

生化/生理作用

变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。

特点和优势

适用于难以裂解的样品。

无微生物DNA，最大限度地减少工作流程中试剂对DNA的污染。

适用于从革兰氏阳性菌中分离核酸，包括李斯特菌、乳酸杆菌和乳球菌。

单位定义

以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600。

外形

冻干粉，含Ficoll® 和琥珀酸钠缓冲盐

法律信息

Ficoll is a registered trademark of Cytiva

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000423551
0000423551
0000400207
0000347115
0000347115

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G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Jennifer A Taylor et al.
Current protocols, 1(4), e80-e80 (2021-04-13)
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2-alkyne muramic acid
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
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