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生物源
bacterial (Streptomyces globisporus)
品質等級
形狀
lyophilized powder
比活性
≥4000 units/mg protein (biuret)
分子量
23 kDa
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成，可能是一种有效的龋病防治剂。
應用
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
诱变溶菌素在一项研究中被用于评估牛奶 链球菌的裂解和原生质体的生成。 它也在一项研究中被用于研究研究B类 链球菌 向原生质体的转化。 它为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
生化／生理作用
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。
單位定義
以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
外觀
冻干粉，含Ficoll® 和琥珀酸钠缓冲盐
其他說明
法律資訊
Fiberfrax is a registered trademark of Unifrax Corp.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Shally R Margolis et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(51) (2021-12-15)
In mammals, cyclic dinucleotides (CDNs) bind and activate STING to initiate an antiviral type I interferon response. CDNs and STING originated in bacteria and are present in most animals. By contrast, interferons are believed to have emerged in vertebrates; thus
实验方案
This procedure may be used for Mutanolysin products.
在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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