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文件

A3547

无色肽酶来源于细菌

Name: 无色肽酶 来源于细菌
Brand: SIGMA

lyophilized powder, ≥1,000 units/mg solid

别名:

ACP, Lysyl endopeptidase

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About This Item

分類程式碼代碼:

12352204

NACRES:

NA.54

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二氧化碳

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生物源

bacterial (LYSOBACTER SP. BACTERIA M497-1)

形狀

lyophilized powder

比活性

≥1,000 units/mg solid

分子量

27 kDa

技術

cell based assay: suitable

適合性

suitable for cell lysis

應用

diagnostic assay manufacturing

運輸包裝

dry ice

儲存溫度

−20°C

應用

消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。

生化／生理作用

消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD₆₀₀=0.6，37℃，超过 2 小时。

消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD₆₀₀=0.6，37℃，超过 2 小时。

單位定義

在 pH8.0，37℃下，使用溶壁微球菌（Micropoccus lysodeikticus）悬浮液作为底物，一个单位的酶每分钟每毫升产生 0.001 的 A₆₀₀ 变化（1cm 光程）。

外觀

粗粉

象形圖

GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P342 + P311

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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实验方案

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

文件

无色肽酶来源于细菌