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CMC0014

Sigma-Aldrich

BL21（DE3）化学感受态细胞

Escherichia coli, rod shaped

别名:

Competent E. coli cells

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.85

product name

BL21（DE3）化学感受态细胞, for protein expression

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

相关类别

一般說明

BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

應用

该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。
  • BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。
  • 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。

    • 特點和優勢

    • 转化效率> 1×107 CFU /µg
    • 实惠的价格
    • 可为日常蛋白表达工作提供卓越价值。

    成分

    • BL21（DE3）化学感受态细胞
    • pUC19转化对照DNA
    • 表达恢复培养基

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    儲存類別代碼

    10 - Combustible liquids

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    BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

    BL21 Chemically Competent Cells Overview

    BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

    BL21 Chemically Competent Cells Overview

    BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

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