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N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

别名:

pNPP tablets

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About This Item

MDL编号:
MFCD00284586
UNSPSC代码:
12352204
NACRES:
NA.83

产品名称

SIGMAFAST™ 对硝基苯基磷酸酯片, tablet, To prepare 5 mL

表单

tablet

溶解性

deionized water: soluble 5 mL

储存温度

−20°C

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相关类别

应用

SIGMA FAST p-磷酸硝基苯酯片剂用于：
  • 定量非分泌型碱性磷酸酶，监测成骨样细胞的分化情况（SaoS-2）
  • 染色小鼠前成骨细胞 MC3T3-E1，进行碱性磷酸酶测定
  • 用作 ExtrAvidin 碱性磷酸酶的酶作用物®，通过酶联免疫吸附法（ELISA）测定血红蛋白结合α-突触核蛋白
  • 用作碱性磷酸酶偶联抗体的酶作用物，进行Abirin毒素的ELISA检测
开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP，0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取，或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水，即可制备活性底物溶液。片剂单独包装在铝箔包装中 。

重悬

将一组片剂溶于5 mL去离子水中，即可得到含有pNPP的即用型缓冲液。

法律信息

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000336007
0000336007
SLCQ9495
0000336006
SLCJ9893

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Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
Nontyphoidal Salmonella (NTS) organisms are a major cause of gastroenteritis and bacteremia, but little is known about maternally acquired immunity and natural exposure in infant populations residing in areas where NTS disease is highly endemic. We recruited 503 pregnant mothers
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Ronit Rosenfeld et al.
Nature communications, 12(1), 944-944 (2021-02-13)
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity and has dramatic consequences on human life, sociality and global economy. Neutralizing antibodies constitute a highly promising
Yuankun Zhai et al.
Journal of cellular physiology, 234(10), 18602-18614 (2019-03-27)
Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet-induced hyperhomocysteinemia (HHcy). However, little is known about Hcy-mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2 S) has potent antioxidant, anti-inflammatory, and antiapoptotic effects. In this
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