The general starting recommendation for conducting immunoprecipitation (IP) using the F3165, anti-FLAG M2 antibody, is 10 μg/mL.
F3165
单克隆抗-FLAG® M2 小鼠抗
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
别名:
抗 ddddk, 抗 dykddddk
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About This Item
UNSPSC代码:
12352203
NACRES:
NA.32
价格与库存信息目前不能提供
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生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin (Purified IgG1 subclass)
抗体产品类型
primary antibodies
克隆
M2, monoclonal
表单
buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
纯化方式
using Protein A
种属反应性
all
浓度
3.8-4.2 mg/mL
技术
western blot: 10 μg/mL (Protein A)
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
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一般描述
抗-Flag M2抗体用于检测Flag融合蛋白质。该单克隆产生于小鼠中并可识别FLAG序列的N-末端、Met N-末端和C-末端。该抗体还可在内部位点识别FLAG。不同于M1，M2不是钙依赖性的。
F3165使用蛋白质A树脂进行亲和纯化，因此不仅含有抗-FLAG M2抗体，还有少量的原生小鼠IgG，这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A
F3165使用蛋白质A树脂进行亲和纯化，因此不仅含有抗-FLAG M2抗体，还有少量的原生小鼠IgG，这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A
免疫原
FLAG；肽序列DYKDDDDK
应用
小鼠产生的 ANTI-FLAG® M2 单克隆抗体已用于：
浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。
- 免疫印迹
- 免疫沉淀
- 免疫细胞化学
- 免疫荧光
- ELISA
- EIA
- 染色质免疫沉淀
- 电子显微分析
- 流式细胞分析
- 高速迁移测定
浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。
制备说明
用3%脱脂牛奶在Tris缓冲盐溶液（TBS），pH 8.0中将抗体从0.5-10 ug/mL进行稀释
储存及稳定性
将稀释后的抗体分装并-20°C储存。不建议反复冻融。
注意：随着时间流逝，由于分子间的疏水相互作用，少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀，请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。
注意：随着时间流逝，由于分子间的疏水相互作用，少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀，请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。
其他说明
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。
相关内容
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
蛋白质与核酸相互作用试剂，以及用于研究蛋白质-RNA、蛋白质-DNA、蛋白质-蛋白质相互作用和相关应用的资源。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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What is the recommended dilution for conducting immunoprecipitation (IP) using the M2 antibody?
1 answer-
Helpful?
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What is the difference between F1804 and F3165 product?
1 answer-
Product F1804 and F3165 are both Monoclonal ANTI-FLAG M2 antibodies produced in mouse Clone M2, with the same starting material. The distinction between the products lies in their purification methods. F3165 is immunoglobulin purified, while F1804 is immunogen affinity purified. F1804, being more purified, is expected to exhibit reduced background and less non-specific staining compared to F3165. Both products are suitable for western blotting, immunofluorescence, and immunoprecipitation.
Helpful?
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What type of light chain is present on the FLAG M2 antibody?
1 answer-
Test has not been conducted to determine whether the light chains of the antibody F3165, Monoclonal ANTI-FLAG® M2 antibody produced in mouse, are kappa or lambda. However, based on customer feedback, it appears that the M2 monoclonal antibody has lambda light chains and not kappa. A probe with anti-kappa did not yield a signal, while anti-lambda produced a good signal.
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