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Merck
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A2220

Millipore

抗-FLAG® M2亲和凝胶

purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗-FLAG® M2亲和琼脂糖凝胶, 抗 ddddk, 抗 dykddddk

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

偶联物

agarose conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

表单

buffered aqueous glycerol solution

分析物化学类别

proteins

技术

affinity chromatography: suitable
immunoprecipitation (IP): suitable

基质

(4% agarose bead; 45-165μm bead size)

同位素/亚型

IgG1

容量

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

抗-FLAG M2亲和凝胶是一种可与琼脂糖共价连接的小鼠单克隆抗体。该抗体可在融合蛋白的N末端、Met-N末端、C末端和内部位置与FLAG结合。结合是不依赖于钙的。

洗脱产物 - FLAG®肽，甘氨酸，pH3.5，3x FLAG®肽
FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide

免疫原

DYKDDDDK

应用

抗-FLAG®M2亲和凝胶已用于western blotting、免疫沉淀以及FLAG融合蛋白的纯化。

请访问我们的FLAG®应用门户网站，了解更多产品详情。

外形

悬浮于含有叠氮化物作为防腐剂以及50％甘油的缓冲盐溶液中

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

FLAG™标签、3x FLAG™、DYKDDDDK标签

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000420716
0000420715
0000420715
0000420716
0000421909

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Nora Nonne et al.
Nucleic acids research, 38(4), e20-e20 (2009-12-04)
MicroRNAs (miRNAs) bind to Argonaute proteins, and together they form the RISC complex and regulate target mRNA translation and/or stability. Identification of mRNA targets is key to deciphering the physiological functions and mode of action of miRNAs. In mammals, miRNAs
Cédric Romilly et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(32), 15901-15906 (2019-07-20)
In bacteria, stable RNA structures that sequester ribosome-binding sites (RBS) impair translation initiation, and thus protein output. In some cases, ribosome standby can overcome inhibition by structure: 30S subunits bind sequence-nonspecifically to a single-stranded region and, on breathing of the
Yu Ti Cheng et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(35), 14694-14699 (2011-08-30)
The nucleotide-binding domain and leucine-rich repeats containing proteins (NLRs) serve as immune receptors in both plants and animals. Overaccumulation of NLRs often leads to autoimmune responses, suggesting that the levels of these immune receptors must be tightly controlled. However, the
Xinna Zhang et al.
The EMBO journal, 30(11), 2177-2189 (2011-04-28)
Tumour suppressor p53 levels in the cell are tightly regulated by controlled degradation through ubiquitin ligases including Mdm2, COP1, Pirh2, and ARF-BP1. The ubiquitination process is reversible via deubiquitinating enzymes, such as ubiquitin-specific peptidases (USPs). In this study, we identified
William G Roach et al.
The Biochemical journal, 403(2), 353-358 (2007-02-06)
Insulin stimulation of the trafficking of the glucose transporter GLUT4 to the plasma membrane is controlled in part by the phosphorylation of the Rab GAP (GTPase-activating protein) AS160 (also known as Tbc1d4). Considerable evidence indicates that the phosphorylation of this
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商品

ANTI-FLAG® M2磁珠

FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

相关内容

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

蛋白纯化

用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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