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形狀
lyophilized powder
品質等級
比活性
≥15 units/mg solid
異物活動
NADH diaphorase activity ≤10 units/mg solid
儲存溫度
−20°C
一般說明
铁氧还蛋白-NADP+ 还原酶是可溶于铁氧还蛋白的配偶子,与PSI的摩尔比为0.9和3。
應用
Ferredoxin-NADP + 还原酶用于 体外 蓝藻类囊体膜中脂肪酸的铁氧化还原蛋白依赖性去饱和作用。也用于调节甘油醛-3-磷酸脱氢酶。
从 Spinacia oleracea(菠菜)中提取的铁氧还蛋白-NADP+ 还原酶用于试验潜在蛋白配偶子对3-磷酸甘油醛脱氢酶(GAPDH)−CP12制剂活性的影响。它也用于标准测定条件,测定藻蓝素:铁氧还蛋白氧化还原酶(PcyA)酶。
生化/生理作用
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族,该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族,该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
單位定義
在菠菜铁氧还蛋白和 NADP 存在的情况下,在 25℃、ph7.5 的条件下,一个单位将每分钟还原 1.0 毫摩尔的细胞色素 C。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
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