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Merck

D5637

Sigma-Aldrich

3,3-二氨基联苯胺 四盐酸盐 水合物

≥96%

别名:

(1,1′-联苯)-3,3′,4,4′-四胺四盐酸盐二水合物

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About This Item

线性分子式:
C12H14N4 · 4HCl · xH2O
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

质量水平

方案

≥96%

表单

powder, crystals or chunks

技术

titration: 96-104% (with NaOH)

颜色

white to light brown, and Faint Red and Faint Grey to Light Grey

mp

280 °C

溶解性

H2O: 50 mg/mL

应用

diagnostic assay manufacturing
hematology
histology

储存温度

−20°C

SMILES字符串

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

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一般描述

3,3′-二氨基联苯胺四盐酸盐水合物是一种常用于免疫组织化学和其他染色技术的显色剂,可在生物样品中可视化抗原或抗体的存在。DAB HCl 设计用作免疫组织学染料,以可视化观测细胞中的过氧化物酶活性。可通过分光光度计测量DAB氧化情况。

应用

3,3′-二氨基联苯胺四盐酸盐水合物适用于免疫组织学染色方案、蛋白质印迹和 FISH(荧光原位杂交)。DAB 用于研究早期白内障时的上皮细胞凋亡,以及常染色体显性遗传性多囊肾 (PKD) 的免疫组织化学方案。

生化/生理作用

在过氧化物存在的条件下,DAB用作过氧化物酶反应中的氢供体。氧化后的DAB形成棕色不溶性复合物。

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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