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Merck

A7595

Sigma-Aldrich

α-淀粉酶 来源于解淀粉芽胞杆菌

greener alternative

liquid, ≥250 units/g

别名:

BAN 480L, 1,4-α-D-葡聚糖葡糖苷水解酶

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About This Item

CAS号:
EC 号:
3.2.1.1.
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形狀

liquid

品質等級

比活性

≥250 units/g

分子量

55 kDa

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

儲存溫度

2-8°C

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一般說明

我们致力于为您带来更加绿色的替代产品,这些产品遵守一项或多项绿色化学12项原则。当用于淀粉乙醇研究时,该产品经优化可提高能效和防止浪费。有关详细信息,请参阅《Biofiles》的文章

應用

α-淀粉酶被用于水解α-连接多糖(如淀粉和糖原)的α键。产品A7595来自于解淀粉芽孢杆菌(Bacillus amyloliquefaciens)并以液体形式提供。α-淀粉酶已用于各种植物研究,如拟南芥(Arabidopsis)的代谢研究。来自解淀粉芽孢杆菌(Bacillus amyloliquefaciens )的α-淀粉酶已用于水解甘薯支链淀粉,以鉴定簇结构。 该酶已被用于将西米棕榈淀粉水解为还原糖,然后将其用于酿酒酵母(Saccharomyces cerevisiae)FNCC 3012的乙醇发酵。该酶能够催化胶凝蜡质玉米淀粉的淀粉分解,产生还原糖。

生化/生理作用

α-淀粉酶是一种内淀粉酶,能够随机水解直链淀粉和支链淀粉中的α-(1,4)-糖苷键。分解产物是具有不同链长度的低聚糖和糊精。该酶在70-90°C的高温下具有最佳活性。
来自解淀粉芽孢杆菌(B.amyloliquefaciens)的α-淀粉酶的pH范围为5.5至6.5,在pH 5.9时具有单一最大值。在低于pH 5.0的条件下,酶迅速失活。
内淀粉酶能够随机水解直链淀粉和支链淀粉中的α-(1→4)-糖苷键。分解产物是具有不同链长度的低聚糖和糊精。该酶在高温下(70−90°C)仍具有活性。

單位定義

一个酶活性单位是指在标准测定条件下,每小时使5.26 g干淀粉糊精化所需的酶量。

法律資訊

Novozyme公司出品。
BAN is a trademark of Novozymes Corp.

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


分析证书(COA)

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Enzymatic and Acid Hydrolysis of Sago Starch for Preparation of Ethanol Production
Sunaryanto R, et al.
Microbiology Indonesia, 7(2), 68-74 (2013)
Fan Zhu et al.
Carbohydrate research, 346(9), 1112-1121 (2011-04-22)
Sweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from Bacillus amyloliquefaciens to release the clusters. Clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. An initial stage of α-amylolysis on
Annabel Bijttebier et al.
Carbohydrate research, 345(2), 235-242 (2009-12-08)
Amylopectin fine structures were studied following limited hydrolysis of gelatinised waxy maize starch by amylases with a different level of inner chain attack (LICA). This was done by size exclusion chromatography as well as by debranching the (partially hydrolysed) amylopectin
Dhananjay Beri et al.
Nature communications, 11(1), 1937-1937 (2020-04-24)
The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology. We report here that Clostridium thermocellum can solubilize over 90% of the carbohydrate in autoclaved corn fiber, including
Kristina J H Kleinwort et al.
Metabolites, 12(3) (2022-03-25)
In recent years, a lack of stability of dairy products with extended shelf life (e.g., yoghurt products, UHT desserts) has occurred, with the corresponding products liquefying significantly after days or weeks. This project aimed to identify the enzymes responsible for

实验方案

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

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